Gram Stain Lab Report
In this section you should describe the results of both qualitative and quantitative methods of analysis. This will be the bulk of your report.
Complete Gram Stain Lab Microbiology Lab Notebook Report Gram Stain Exercise 3 February 6 2020 Studocu
Using a sterile 5 mL pipette add 5mL of sterile saline to a sterile.
. Gram cfug of test material. To confirm culture purity. Please consult your doctor or the laboratory that performed the tests to obtain the reference range if you do not have the lab report.
A bacterial wound culture is primarily used along with a Gram stain and other tests to help determine whether a wound is infected and to identify the bacteria causing the infection. Make sure data is both graphed or diagrammed as well as described. StaphsStreps Gram Enterics Gram- Motility.
This complex is a larger molecule than the original crystal violet stain and iodine and is insoluble in water. Because we generally have no idea of how many bacteria are in a sample it is almost always necessary to prepare a dilution series to ensure that we obtain a dilution containing a reasonable number of bacteria to count. Designate a section of your report for your results.
This lab should help give you the background information and techniques you will need to successfully perform general biochemical tests in order to help identify unknown. All graphs and diagrams should have a number and title. Aureus is a gram positive bacterium that when looked at under a microscope it appears to be a cluster of what.
A gram stain was taken from a sample of the bacteria growing on the nutrient agar. Results obtained by culture without evaluation for contamination may be noncontributory or misleading. The bacterias form describes how they spread in a petri dish and can be.
Dilutions in the range 10-1 110 to 10-8 range of dilutions can be restricted. SYTO 9 green fluorescent nucleic acid stain has been shown to stain live and dead Gram-positive and Gram-negative bacteria and it is a component of the LIVEDEAD BacLight Bacterial Viability Kits L-7007 L-7012 L-13152. The results showed the nutrient agar was only allowing one bacteria to grow.
For these reasons you will not find reference ranges for the majority of tests described on this web site. It represents one aspect of water qualityIt is a microbiological analytical procedure which uses samples of water and from these samples determines the concentration of bacteria. It is then possible to draw inferences about.
When doing a gram stain the results showed that the bacterium was Gram negative rods. All sections including Intro Results Methods and Conclusion. 1 A Gram stain from a carefully collected specimen with neutrophils and lancet-shaped diplococci staining gram-positive which are intracellular or encapsulated can provide strong support to the clinical diagnosis of pneumococcal pneumonia.
If a culture reveals that a wound is infected susceptibility testing is done to determine which antibiotic will inhibit the growth of the bacteria causing the infection. Stab with a needle straight in and straight out of the center of the tube half. The gram stain turned out to be a positive rod gram stain.
Common physical characteristics of bacteria colonies are listed and separated into 3 categories. This is because abnormally high or low albumin concentrations can alter total calcium concentration independently of ionised active calcium concentration. Gram staining differentiates bacteria by the chemical and physical properties of their.
Perform a Gram Stain to confirm culture purity from your subculture plate. Bacteriological water analysis is a method of analysing water to estimate the numbers of bacteria present and if needed to find out what sort of bacteria they are. The lab report containing your test results should include the relevant reference range for your tests.
Transport storage waste disposal Gram stain microscopy biochemistry measurements culture identification antimicrobial susceptibility testing typing methods serological or molecular. Example Lab Report of Staphylococcus aureus. Circular covering the whole dish irregular spreading out in a non-uniform pattern filamentous spreading out like roots towards the outer edge and rhizoid spreading out like branches with main segments.
A Mannitol Salt Agar plate was obtained in order to test for mannitol fermentation. Most commonly laboratories report a value for adjusted calcium also known as corrected calcium which is the measured calcium value adjusted for the albumin concentration. The information presented in this lab is from The Manual of Clinical Microbiology 8th Ed.
Request forms report forms and other laboratory forms are all important components of the quality manual which documents the quality management system. Conversely the the outer membrane of Gram negative bacteria is degraded and the thinner peptidoglycan layer of Gram negative cells is unable to retain the crystal violet-iodine complex and the color is lost. Gram stain or Gram staining also called Grams method is a method of staining used to classify bacterial species into two large groups.
Here is a good Example of an Unknown Lab Report in Microbiology. Gram-positive bacteria and gram-negative bacteriaThe name comes from the Danish bacteriologist Hans Christian Gram who developed the technique in 1884. The procedures are paraphrased from the National Committee for Clinical Laboratory Standards NCCLS 2000.
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